Coding

Part:BBa_K4865002:Design

Designed by: Jing Shi   Group: iGEM23_OTIA-Hangzhou   (2023-08-24)


PelB-EGF-R


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 54
    Illegal AgeI site found at 328
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 208


Design Notes

The PelB coding sequence has a start codon, while other coding sequences do not.


Source

PelB from Bacillus sp., EGF from Homo sapiens and PySp1 from Araneus ventricosus.


References